Matrix Assisted Laser Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) allows analysis of proteins and peptides with high sensitivity and soft ionization with little or no fragmentation. Ions are formed through gas-phase proton-transfer reactions with photoionized matrix molecules. Matrix is typically a small energy absorbing molecule such as alfa-cyano-4-hydroxycinnamic acid.
MALDI-TOF MS analysis produces data for protein/peptide molecular weight determination as well as protein identification by peptide mass fingerprinting.
Example MALDI-TOF linear mass spectrum of intact protein: Hg-Papain oligomerization
Example MALDI-TOF reflector mass spectrum of digested protein: Advanced glycosylation end product-specific receptor was identified by peptide mass fingerprinting database search
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